Chromatography as a Separation Technique


Chromatography is a method of separating compounds from a mixture. The technique is both preparative, analytical and is employed widely in laboratory as well as industrial standards. More than 70% of chemical analysis all over the world is done using Chromatography. It is a physical technique applied from basic organic chemistry to Forensic sciences. Some of the common applications include detection of cocaine in urine, alcohol in blood, PCB’s in fish, lead in water, etc. Chromatography is of various types-displacement chromatography, Paper chromatography, Thin layer chromatography, super critical chromatography, expanded bed adsorption chromatography, liquid chromatography, Gas chromatography, Column fluid chromatography in various combinations.

·              Advancements in chromatography techniques

·              Molecular exclusion as separation technique

·              HPLC & UHPLC as a separation technique

·              Use of chromatography in toxicology

·              Gas chromatography

·              Chiral chromatography

·              Affinity chromatography

·              TLC & HPTLC

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